Predictive biomarkers of drug response in Triple negative breast cancer


Triple negative breast cancers (TNBCs) lack Estrogen receptor, Progesterone receptor and Epidermal growth factor receptor 2 (HER2) and accounts for approximately 10-20% of breast cancers. They are common in women under 40 and black women and are characterized by poor prognosis. The current treatment remains surgery, chemotherapy, and radiotherapy, but is associated with high relapse risk. This necessitates the need for novel therapeutic approaches 1.

A large proportion (50-75%) of TNBCs express Epidermal Growth Factor Receptor (EGFR). However, clinical trials with EGFR targeting studies have not been promising. A recent study by Foidart et al2, uncovers an interesting loop involving three genes - EGFR, membrane-type-4 matrix metalloproteinase (MT4-MMP), and retinoblastoma protein (RB) in TNBCs which could be therapeutically exploited in the management of the disease. Their studies which were conducted in in human breast tumour specimens, xenografts, and  patient derived TNBCs (PDX-TNBC) xenografts  reveal that approximately half of all TNBC and PDX-TNBC express the three genes. More than 70% of TNBC samples and PDX-TNBC co-express EGFR and MT4-MMP, and approximately 60% express RB.

Experiments on TNB cells coexpressing EGFR and MT4-MMP show that single erlotinib and palbociclib treatments radically reduce proliferation when compared with control cells. In the xenografts, those coexpressing MT4-MMP, EGFR, and RB show sensitivity to erlotinib and palbociclib individually, and display an additive effect when administered together. This phenomenon is not seen  in xenografts that lack Rb.

This study crucially demonstrates the efficacy of erlotinib–palbociclib combination in MT4-MMP/EGFR/RB tumors that represent 50% of TNBC patients, and shows that a subgroup of patients who could be successfully treated with EGFR and CDK4/6 inhibitors can be identified by profiling the tumours for the three biomarkers which allows for effective patient selection and targeted therapy.

References:


2.http://clincancerres.aacrjournals.org/content/25/6/1838